Detailed view for LmjF.26.0310
Basic information
Leishmania major, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-1-like protein
Source Database / ID: TriTrypDB GeneDB
pI: 5.8944 |
Length (AA): 667 |
MW (Da): 74268 |
Paralog Number:
0
Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0
Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable
Network
Pfam domains
Gene Ontology
GO:0005524 ATP binding
GO:0005515 protein binding
GO:0003873 6-phosphofructo-2-kinase activity
GO:0003824 catalytic activity
GO:0006000 fructose metabolic process
Metabolic Pathways
Structural information
Modbase 3D models:
There are 15 models calculated for this protein. More info on
these models, including the
models themselves is available at:
Modbase
| Target Beg | Target End | Template | Template Beg | Template End | Identity | Evalue | Model Score | MPQS | zDope |
|---|---|---|---|---|---|---|---|---|---|
| 16 | 175 | 1svx (A) | 21 | 165 | 27.00 | 0 | 1 | 0.55 | -0.52 |
| 93 | 175 | 1svx (A) | 17 | 99 | 40.00 | 0.0000000013 | 1 | 0.82 | -2.03 |
| 193 | 654 | 2axn (A) | 5 | 436 | 27.00 | 0 | 1 | 1.02 | -0.83 |
| 217 | 664 | 1k6m (A) | 39 | 451 | 28.00 | 0 | 1 | 1.04 | -1.11 |
| 10 | 172 | 5eil (A) | 3 | 151 | 31.00 | 0 | 0.89 | 0.525578 | 0.08 |
| 11 | 173 | 4rlv (A) | 2596 | 2778 | 21.00 | 0.00000011 | 1 | 0.397578 | 0.27 |
| 11 | 167 | 1k1a (A) | 166 | 312 | 21.00 | 0.0021 | 0.47 | 0.396582 | 0.06 |
| 23 | 172 | 4k5c (A) | 28 | 176 | 25.00 | 0.000000000042 | 0.99 | 0.602088 | -0.38 |
| 53 | 185 | 2bkg (A) | 17 | 141 | 36.00 | 0.00000000024 | 1 | 0.6944 | -1.04 |
| 93 | 175 | 2xen (A) | 17 | 99 | 39.00 | 0.00000000058 | 1 | 0.747438 | -1.42 |
| 98 | 184 | 1n0r (A) | 10 | 93 | 44.00 | 0.000000000042 | 1 | 0.720435 | -0.89 |
| 187 | 656 | 5ak0 (A) | 1 | 439 | 22.00 | 0 | 1 | 0.900448 | 0.02 |
| 220 | 665 | 1k6m (A) | 42 | 453 | 27.00 | 0 | 1 | 0.970466 | -0.42 |
| 221 | 658 | 1k6m (A) | 43 | 443 | 31.00 | 0 | 1 | 0.939672 | -0.12 |
| 431 | 505 | 3f2i (A) | 1 | 73 | 24.00 | 0 | 0.07 | 0.262244 | 0.14 |
Help me make sense of these data.
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)
A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.
PDB Structures:
Expression
| Upregulation Percent | Ranking | Stage | Dataset |
|---|---|---|---|
| Upper 60-80% percentile | amastigotes, metacyclic. | Fernandes MC |
-
Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.
Orthologs
Ortholog group members (OG5_147018)
| Species | Accession | Gene Product |
|---|---|---|
| Leishmania braziliensis | LbrM.26.0340 | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-1-like protein |
| Leishmania donovani | LdBPK_260300.1 | 6-phosphofructo-2-kinase/fructose-2,6-biphospha ta se1-like protein |
| Leishmania infantum | LinJ.26.0300 | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-1-like protein |
| Leishmania major | LmjF.26.0310 | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-1-like protein |
| Leishmania mexicana | LmxM.26.0310 | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase-1-like protein |
| Trypanosoma brucei gambiense | Tbg972.7.1620 | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase, putative |
| Trypanosoma brucei | Tb927.7.1610 | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase, putative |
| Trypanosoma congolense | TcIL3000_7_1160 | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase, putative |
| Trypanosoma cruzi | TcCLB.508207.230 | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase, putative |
| Trypanosoma cruzi | TcCLB.509509.30 | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase, putative |
Essentiality
| Gene/Ortholog | Organism | Phenotype | Source Study |
|---|---|---|---|
| Tb927.7.1610 | Trypanosoma brucei | no significant loss or gain of fitness in bloodstream forms (3 days) | alsford |
| Tb927.7.1610 | Trypanosoma brucei | significant gain of fitness in bloodstream forms (6 days) | alsford |
| Tb927.7.1610 | Trypanosoma brucei | no significant loss or gain of fitness in procyclic forms | alsford |
| Tb927.7.1610 | Trypanosoma brucei | significant gain of fitness in differentiation of procyclic to bloodstream forms | alsford |
-
blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930 -
keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection -
neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs -
nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource -
wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170 -
gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84 -
alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924 -
yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database -
shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
Phenotypes and Validation (curated)
In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
In any case, if you have information about papers containing relevant validation data for this target, please contact us.
Annotated validation
Associated compounds / Druggability
Known modulators for this target
Predicted associations
By orthology with druggable targets
By sequence similarity to non orthologous druggable targets
Obtained from network model
Ranking Plot
Putative Drugs List
Assayability
Assay information
- BRENDA Assay
- An enzyme with this EC number or name or sequence has been assayed in Leishmania major ( 1 )
Reagent availability
- Reagent:
-
Target Type Source Notes LmjF.26.0310 cloned gene BRENDA A gene with this EC number or name or sequence has been cloned from Leishmania major ( 2 )
Bibliographic References